PREPARATION OF 3'-PHOSPHOADENOSINE-5'-PHOSPHO S-35 SULFATE USING ATP SULFURYLASE AND APS KINASE FROM BACILLUS-STEAROTHERMOPHILUS - ENZYMATIC-SYNTHESIS AND PURIFICATION

被引：12

作者：

HIRAM, P

FERNANDO, P

KARAKAWA, A

SAKAKIBARA, Y

IBUKI, H

NAKAJIMA, H

LIU, MC

SUIKO, M

机构：

[1] UNITIKA RES & DEV CTR, UJI, KYOTO 611, JAPAN

[2] UNIV TEXAS, CTR HLTH, DEPT BIOCHEM, TYLER, TX 75710 USA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期

关键词：

D O I：

10.1271/bbb.57.1974

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radioactive PAPS was synthesized with a predetermined specific activity using enzymes purified from Bacillus stearothermophilus. The reaction system, incorporating 1.5 mCi of S-35-H2SO4 and 500 nmol of ATP, produced 582 muCi of S-35-PAPS. The synthesized S-35-PAPS was purified by ECTEOLA cellulose chromatography, followed by desalting with DOWEX 50W-X8. The final yield was 206 mumol of S-35-PAPS with a radioactivity of 411 muCi.

引用

页码：1974 / 1975

页数：2

共 7 条

[1] AN ECTEOLA-CELLULOSE CHROMATOGRAPHY ASSAY FOR 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE - PHENOL SULFOTRANSFERASE [J].