MONOCLONAL-ANTIBODIES AGAINST HUMAN FIBROBLAST COLLAGENASE AND THE DESIGN OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY TO MEASURE TOTAL COLLAGENASE

被引：43

作者：

CLARK, IM

POWELL, LK

WRIGHT, JK

CAWSTON, TE

HAZLEMAN, BL

机构：

[1] Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, CB2 2QQ, Hills Road

来源：

MATRIX | 1992年 / 12卷 / 06期

关键词：

COLLAGENASE; ENZYME IMMUNOASSAY; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP);

D O I：

10.1016/S0934-8832(11)80092-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies have been raised against purified human fibroblast collagenase and characterised. One of these antibodies has been used in combination with a polyclonal anticollagenase antibody in a double antibody sandwich ELISA to measure collagenase. The assay range was 2-50 ng/ml collagenase. The assay measures total collagenase, i.e. pro-and active enzyme as well as collagenase in complex with TIMP. The level of collagenase has been measured in sera samples from patients with rheumatoid arthritis and compared with age- and sex-matched controls. The levels measured were: rheumatoid arthritis, 69 +/- 29 ng/ml; normal, 49 +/- 14 ng/ml.

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页码：475 / 480

页数：6

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