LINKAGE OF VARIABLE END OF BACTERIOPHAGE MU DNA TO TAIL

被引：8

作者：

BREEPOEL, H

HOOGENDORP, J

MELLEMA, JE

WIJFFELMAN, C

机构：

[1] STATE UNIV LEIDEN, GENET LAB, LEIDEN, NETHERLANDS

[2] STATE UNIV LEIDEN, DEPT BIOCHEM, LEIDEN, NETHERLANDS

来源：

VIROLOGY | 1976年 / 74卷 / 02期

关键词：

D O I：

10.1016/0042-6822(76)90335-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteriophage Mu particles were disrupted by treatment at high pH and the DNA released from the phage was analyzed by EM. The DNA appeared to be associated with the proximal end of the tail. Denaturation maps of Mu DNA were obtained by heating and UV irradiation. The profiles of the 2 maps were comparable and indicated that the same end of the Mu genome was always connected to the sheath structure. Analysis of the DNA fragments after treatment of the DNA-tail complex with the endonuclease Eco R1 showed that the variable end of the Mu DNA was attached to the tail.

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页码：279 / 286

页数：8

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