VOLTAGE-GATED K+ CURRENTS OF MOUSE DENDRITIC CELLS

被引：22

作者：

FISCHER, HG

EDER, C

机构：

[1] HUMBOLDT UNIV BERLIN,INST PHYSIOL CHARITE,NEUROPHYSIOL ABT,D-10117 BERLIN,GERMANY

[2] UNIV DUSSELDORF,INST MED MIKROBIOL & VIROL,D-40225 DUSSELDORF,GERMANY

来源：

FEBS LETTERS | 1995年 / 373卷 / 02期

关键词：

DENDRITIC CELL; MHC CLASS II; ANTIGEN PRESENTATION; PATCH CLAMP; POTASSIUM CURRENT;

D O I：

10.1016/0014-5793(95)01029-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dendritic cells (DC) were enriched from murine spleen by exploiting their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofunctional characteristics of DC, Cells of typical dendritic shape mere electrophysiologically analysed using the whole cell. configuration of the patch clamp technique, All 26 cells expressed only outward K+ currents comparable to those detected in cytokine-activated microglia, Co-purified splenic macrophages, in contrast, displayed an inward rectifying K+ current.

引用

页码：127 / 130

页数：4

共 15 条

[1]

EDER C, IN PRESS J MEMBR BIO

[2] DIFFERENTIATION DRIVEN BY GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR ENDOWS MICROGLIA WITH INTERFERON-GAMMA-INDEPENDENT ANTIGEN PRESENTATION FUNCTION [J].