INTERFERON ACTIVATION OF THE TRANSCRIPTION FACTOR STAT91 INVOLVES DIMERIZATION THROUGH SH2-PHOSPHOTYROSYL PEPTIDE INTERACTIONS

被引：716

作者：

SHUAI, K ^{[1
]}

HORVATH, CM ^{[1
]}

HUANG, LHT ^{[1
]}

QURESHI, SA ^{[1
]}

COWBURN, D ^{[1
]}

DARNELL, JE ^{[1
]}

机构：

[1] ROCKEFELLER UNIV, PLANT BIOCHEM LAB, NEW YORK, NY 10021 USA

来源：

CELL | 1994年 / 76卷 / 05期

关键词：

D O I：

10.1016/0092-8674(94)90357-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stat91 (a 91 kd protein that acts as a signal transducer and activator of transcription) is inactive in the cytoplasm of untreated cells but is activated by phosphorylation on tyrosine in response to a number of polypeptide ligands, including interferon alpha (IFN-alpha) and lFN-gamma. We report here that the inactive Stat91 in the cytoplasm of untreated cells is a monomer and that upon IFN-gamma-induced phosphorylation it forms a stable homodimer. Only the dimer is capable of binding to a specific DNA sequence directing transcription. Through dissociation and reassociation assays, we show that dimerization of Stat91 is mediated through SH2-phosphotyrosyl peptide interactions. Dimerization involving SH2 recognition of specific phosphotyrosyl peptides may well provide a prototype for interactions among family members of STAT proteins to form different transcription complexes.

引用

页码：821 / 828

页数：8

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