RAPID ANALYSIS OF MOUSE - HAMSTER HYBRID CELL-LINES BY INSITU HYBRIDIZATION

被引：25

作者：

BOYLE, AL ^{[1
]}

LICHTER, P ^{[1
]}

WARD, DC ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510

来源：

GENOMICS | 1990年 / 7卷 / 01期

关键词：

D O I：

10.1016/0888-7543(90)90529-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In situ htbridization techniques for analyzing the murine DNA complement of mouse-hamster hybrid cells are described. Total genomic mouse DNA is labeled with biotin and hybridized without suppression to metaphase spreads from a mouse-hamster hybrid line containing the mouse fusion chromosome X12. Detection via fluorochrome-conjugated avidin reveals mouse chromosomal DNA with high sensitivity and permits the identification of both normal and aberrant murine chromosomes. Conversely, biotinylated total genomic DNA from a hybrid line can be used as a probe on normal mouse metaphase spreads if suppression techniques are employed, facilitating the analysis of mouse chromosomes present in the hybrid line. © 1990.

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页码：127 / 130

页数：4

相关论文

共 17 条

[1] DIFFERENTIAL STAINING OF HUMAN AND MOUSE CHROMOSOMES IN INTERSPECIFIC CELL HYBRIDS [J].

BOBROW, M ;

CROSS, J .

NATURE, 1974, 251 (5470) :77-79

[2] DETECTION OF CHROMOSOME-ABERRATIONS IN METAPHASE AND INTERPHASE TUMOR-CELLS BY INSITU HYBRIDIZATION USING CHROMOSOME-SPECIFIC LIBRARY PROBES [J].

CREMER, T ;

LICHTER, P ;

BORDEN, J ;

WARD, DC ;

MANUELIDIS, L .

HUMAN GENETICS, 1988, 80 (03) :235-246

[3] DETECTION OF SPECIES-SPECIFIC CHROMOSOMES IN SOMATIC-CELL HYBRIDS [J].

DURNAM, DM ;

GELINAS, RE ;

MYERSON, D .

SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (06) :571-577

[4] STAINING OF CONSTITUTIVE HETEROCHROMATIN IN MAMMALIAN CHROMOSOMES WITH A NEW FLUOROCHROME [J].

HILWIG, I ;

GROPP, A .

EXPERIMENTAL CELL RESEARCH, 1972, 75 (01) :122-&

[5] SEQUENTIAL STAINING TECHNIQUE FOR CHROMOSOMAL ANALYSIS OF INTERSPECIFIC MOUSE-HAMSTER AND MOUSE-HUMAN SOMATIC-CELL HYBRIDS [J].

KOZAK, CA ;

LAWRENCE, JB ;

RUDDLE, FH .

EXPERIMENTAL CELL RESEARCH, 1977, 105 (01) :109-117

[6] USE OF WHOLE COSMID CLONED GENOMIC SEQUENCES FOR CHROMOSOMAL LOCALIZATION BY NON-RADIOACTIVE INSITU HYBRIDIZATION [J].

LANDEGENT, JE ;

DEWAL, NJI ;

DIRKS, RW ;

BAAS, F ;

VANDERPLOEG, M .

HUMAN GENETICS, 1987, 77 (04) :366-370

[7] DELINEATION OF INDIVIDUAL HUMAN-CHROMOSOMES IN METAPHASE AND INTERPHASE CELLS BY INSITU SUPPRESSION HYBRIDIZATION USING RECOMBINANT DNA LIBRARIES [J].

LICHTER, P ;

CREMER, T ;

BORDEN, J ;

MANUELIDIS, L ;

WARD, DC .

HUMAN GENETICS, 1988, 80 (03) :224-234

[8] HELPER-INDEPENDENT TRANSFORMATION BY UNINTEGRATED HARVEY SARCOMA-VIRUS DNA [J].

LOWY, DR ;

RANDS, E ;

SCOLNICK, EM .

JOURNAL OF VIROLOGY, 1978, 26 (02) :291-298

[9] INDIVIDUAL INTERPHASE CHROMOSOME DOMAINS REVEALED BY INSITU HYBRIDIZATION [J].

MANUELIDIS, L .

HUMAN GENETICS, 1985, 71 (04) :288-293

[10] A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES [J].

MOYZIS, RK ;

BUCKINGHAM, JM ;

CRAM, LS ;

DANI, M ;

DEAVEN, LL ;

JONES, MD ;

MEYNE, J ;

RATLIFF, RL ;

WU, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6622-6626