ANALYSIS OF BORDETELLA-PERTUSSIS CYA OPERON REGULATION BY USE OF CYA-LAC FUSIONS

被引：26

作者：

GOYARD, S ^{[1
]}

ULLMANN, A ^{[1
]}

机构：

[1] INST PASTEUR,DEPT BIOCHIM & GENET MOLEC,UNITE BIOCHIM REGULAT CELLULAIRES,F-75724 PARIS 15,FRANCE

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 77卷 / 2-3期

关键词：

ADENYLATE CYCLASE; PHENOTYPIC MODULATION; VIRULENCE REGULATION;

D O I：

10.1111/j.1574-6968.1991.tb04357.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neither is it activated by bvg, when introduced in trans. The cya-lac fusion is fully active when returned to B. pertussis by homologous recombination and responds to bvg-dependent activation and environmental regulation. These results indicate that in B. pertussis the activation of the cya operon by bvg is indirect.

引用

页码：251 / 256

页数：6

共 19 条

[1] SEQUENCES REQUIRED FOR EXPRESSION OF BORDETELLA-PERTUSSIS VIRULENCE FACTORS SHARE HOMOLOGY WITH PROKARYOTIC SIGNAL TRANSDUCTION PROTEINS [J].