A GENE PAIR FROM THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX ENCODES LARGE PROLINE-RICH PROTEINS WITH MULTIPLE REPEATED MOTIFS AND A SINGLE UBIQUITIN-LIKE DOMAIN

A large number of genes has been identified previously between the class I and class II gene families within the class III region of the human major histocompatibility complex. The complete sequences of two of these genes, BAT2 and BAT3 (where BAT is HLA-B-associated transcript), which are closely linked, were determined from cDNA clones. The putative BAT2 and BAT3 proteins are 228 and 110 kDa, respectively, and do not appear to be members of any known family of proteins. However, BAT3 contains an amino-terminal ubiquitin-like domain. Both BAT2 and BAT3 are very rich in proline and include short tracts of polyproline, polyglycine, and charged amino acids. In addition, these proteins contain several unrelated families of similar repeated segments. BAT2 and BAT3 are similar to other proteins with large proline-rich domains, such as some nuclear proteins, collagens, elastin, and synapsin. BAT2 also contains four Arg-Gly-Asp (RGD) motifs typical of the integrin receptor family.