HYBRIDOMA MONOCLONAL-ANTIBODIES TO HUMAN-FETAL HEMOGLOBIN

被引:12
作者
CUCINA, A
REVOLTELLA, RP
MANCINI, R
DONOFRIO, G
机构
[1] CNR, INST CELL BIOL, VIA GD ROMAGNOSI 18A, I-00196 ROME, ITALY
[2] UNIV CATTOLICA SACRO CUORE, INST CELL BIOL, I-00168 ROME, ITALY
关键词
D O I
10.1111/j.1365-3083.1984.tb00935.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Monoclonal antibodies against human fetal haemoglobin (MbF) were obtained by fusion of mouse myeloma cells with spleen lymphoeyies from mice immunized against HbF from human umbilical cord blood erythrocyles. Initial antibody screening was by indirect immunofluor‐escence assays on smeared cord blood and normal adult blood erythrocytes. The specificity of these antibodies was further studied by combined immunoprecipitation assays and direct or inhibition solid‐phase enzyme immunoassays. The combined pattern enabled recognition of three types of antigenic determinants: (i) common to both HbF and HbA, by both immunofluroescence and enzyme immunoassays; (ii) common to both HbF and HbA by enzyme immunoassays, present on fixed cord blood erythrocytes and adult thalassaemic erythrocytes by immunofluorcscence, but not found on fixed normal adult erythrocytes; and (iii) private HbF by both immunofluorescence and enzyme immunoassays. Copyright © 1984, Wiley Blackwell. All rights reserved
引用
收藏
页码:307 / 313
页数:7
相关论文
共 9 条
[1]   ESTIMATION OF SMALL PERCENTAGES OF FOETAL HAEMOGLOBIN [J].
BETKE, K ;
MARTI, HR ;
SCHLICHT, I .
NATURE, 1959, 184 (4702) :1877-1878
[2]   STUDIES ON HETEROGENEITY OF HEMOGLOBIN .13. CHROMATOGRAPHY OF VARIOUS HUMAN AND ANIMAL HEMOGLOBIN TYPES ON DEAE-SEPHADEX [J].
DOZY, AM ;
KLEIHAUER, EF ;
HUISMAN, THJ .
JOURNAL OF CHROMATOGRAPHY, 1968, 32 (04) :723-+
[3]   SIMPLE METHOD FOR POLYETHYLENE GLYCOL-PROMOTED HYBRIDIZATION OF MOUSE MYELOMA CELLS [J].
GEFTER, ML ;
MARGULIES, DH ;
SCHARFF, MD .
SOMATIC CELL GENETICS, 1977, 3 (02) :231-236
[4]  
HUISMAN JHJ, 1977, HEMOGLOBINOPATHIES T, P69
[5]   CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].
KOHLER, G ;
MILSTEIN, C .
NATURE, 1975, 256 (5517) :495-497
[6]   [I125] PROTEIN-A - TRACER FOR GENERAL USE IN IMMUNOASSAY [J].
LANGONE, JJ .
JOURNAL OF IMMUNOLOGICAL METHODS, 1978, 24 (3-4) :269-285
[7]   RAPID ELECTROPHORESIS AND QUANTITATION OF HAEMOGLOBINS ON CELLULOSE ACETATE [J].
MARENGOROWE, AJ .
JOURNAL OF CLINICAL PATHOLOGY, 1965, 18 (06) :790-+
[8]   RECENT DEVELOPMENTS IN THE MOLECULAR GENETICS OF HUMAN-HEMOGLOBIN [J].
WEATHERALL, DJ ;
CLEGG, JB .
CELL, 1979, 16 (03) :467-479
[9]  
Wood W G, 1977, Prog Hematol, V10, P43