FURTHER STUDY ON REACTION PRODUCTS OF PRYRIDOXAL AND PYRIDOXAL 5'-PHOSPHATE WITH CYANIDE

被引:26
作者
OHISHI, N
FUKUI, S
机构
[1] Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Kyoto University, Kyoto
关键词
D O I
10.1016/0003-9861(68)90070-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reaction products of cyanide with pyridoxal and with pyridoxal 5′-phosphate were reexamined. Under weak alkaline conditions employed in the fluorometric assay of these compounds, they were converted almost quantitatively to a product having strong fluorescence. The results of ultraviolet, infrared, and fluorescence spectrophotometry, paper chromatography, paper electrophoresis, and other tests confirm the identity of the reaction products as 4-pyridoxic acid lactone and 4-pyridoxic acid 5′-phosphate, respectively. © 1968.
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页码:606 / &
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