CLONING OF A TRANSCRIPTIONALLY ACTIVE HUMAN TATA BINDING-FACTOR

被引：301

作者：

KAO, CC

LIEBERMAN, PM

SCHMIDT, MC

ZHOU, Q

PEI, R

BERK, AJ

机构：

[1] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, DEPT MICROBIOL, LOS ANGELES, CA 90024 USA

[2] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, DEPT BIOL CHEM, LOS ANGELES, CA 90024 USA

来源：

SCIENCE | 1990年 / 248卷 / 4963期

关键词：

D O I：

10.1126/science.2194289

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcrption factor IID (TFIID) binds to the TATA box promoter element and regulates the expression of most eukaryotic genes transcribed by RNA polymerase II. Complementary DNA (cDNA) encoding a human TFIID protein has been cloned. The human TFIID polypeptide has 339 amino acids and a molecular size of 37,745 daltons. The carboxyl-terminal 181 amino acids of the human TFIID protein shares 80% identity with the TFIID protein from Saccharomyces cerevisiae. The amino terminus contains an unusual repeat of 38 consecutive glutamine residues and an X-Thr-Pro repeat. Expression of DNA in reticulocyte lysates or in Escherichia coli yielded a protein that was competent for both DNA binding and transcription activation.

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页码：1646 / 1650

页数：5

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