REGULATION OF CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT (CINC) MESSENGER-RNA PRODUCTION IN CULTURED RAT-CELLS

被引：8

作者：

DOLECKI, GJ ^{[1
]}

DELARCO, JE ^{[1
]}

机构：

[1] MONSANTO CO,MONSANTO CORP RES,DIV HLTH SCI,ST LOUIS,MO 63167

来源：

DNA AND CELL BIOLOGY | 1994年 / 13卷 / 09期

关键词：

D O I：

10.1089/dna.1994.13.883

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat cytokine-induced neutrophil chemoattractant (CINC) is an 8-kD polypeptide originally purified from media conditioned by interleukin-1 beta (IL-1 beta)-stimulated 52E, an epithelioid clone derived from the normal rat kidney (NRK) cell line. Using a fibroblastic clone of NRK cells, 49F, we found that lipopolysaccharide (LPS), IL-1 beta, and tumor necrosis factor-alpha (TNF-alpha) each induce synthesis of CINC mRNA and CINC, although in qualitatively and quantitatively different patterns. Through deadenylation experiments and by probing with oligonucleotides, we discovered that the smaller of the two major CINC transcripts appears to arise from the larger as a result of poly(A) tail removal and/or 3' cleavage.

引用

页码：883 / 889

页数：7

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