OXIDATIVE DNA-DAMAGE INDUCED BY SIMULTANEOUS GENERATION OF NITRIC-OXIDE AND SUPEROXIDE

被引：323

作者：

INOUE, S ^{[1
]}

KAWANISHI, S ^{[1
]}

机构：

[1] KYOTO UNIV,GRAD SCH MED,DEPT PUBL HLTH,KYOTO 606,JAPAN

来源：

FEBS LETTERS | 1995年 / 371卷 / 01期

关键词：

8-HYDROXYDEOXYGUANOSINE; 3-MORPHOLINOSYDNONIMINE; NITRIC OXIDE; SUPEROXIDE; OXIDATIVE DNA DAMAGE; PEROXYNITRITE;

D O I：

10.1016/0014-5793(95)00873-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of calf thymus DNA with 3-morpholinosydnonimine (SIN-1), which simultaneously generates nitric oxide (NO) and superoxide (O-2(-)), induced a significant increase of 8-hydroxydeoxyguanosine (8-OH-dG). Peroxynitrite also increased 8-OH-dG in calf thymus DNA. Addition of free hydroxyl radical (. OH) scavengers inhibited the increase of 8-OH-dG by SIN-1 or peroxynitrite. Incubation of P-32-labeled DNA fragment with SIN-1 or peroxynitrite caused DNA cleavage at every nucleotide with a little dominance at guanine residues. The results suggest that NO reacts with O-2(-) to form peroxynitrite and the peroxynitrite induces oxidative DNA damage through an active intermediate of which reactivity is similar to . OH.

引用

页码：86 / 88

页数：3

共 23 条

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