A HOMOLOGY MODEL OF HUMAN INTERFERON-ALPHA-2

被引：30

作者：

MURGOLO, NJ ^{[1
]}

WINDSOR, WT ^{[1
]}

HRUZA, A ^{[1
]}

REICHERT, P ^{[1
]}

TSARBOPOULOS, A ^{[1
]}

BALDWIN, S ^{[1
]}

HUANG, E ^{[1
]}

PRAMANIK, B ^{[1
]}

EALICK, S ^{[1
]}

TROTTA, PP ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT BIOCHEM & MOLEC & CELL BIOL,ITHACA,NY 14853

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1993年 / 17卷 / 01期

关键词：

ALPHA-INTERFERON; INTERFERON-BETA; HOMOLOGY MODELING;

D O I：

10.1002/prot.340170109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An atomic coordinate five alpha-helix three-dimensional model is presented for human interferon alpha-2 (HuIFNalpha2). The HuIFNalpha2 structure was constructed from murine interferon beta (MuIFNbeta) by homology modeling using the STEREO and IMPACT programs. The HuIFNalpha2 model is consistent with its known biochemical and biophysical properties including epitope mapping. Lysine residues predicted to be buried in the model were primarily unreactive with succinimidyl-7-amino-4-methylcoumarin-3-acetic acid (AMCA-NHS), a lysine modification agent, as shown by mass spectrometric analysis of tryptic digests. N-terminal sequence analysis of polypeptides generated by limited digestion of HuIFNalpha2 with endoproteinase Lys-C demonstrated rapid cleavage at K31, which is consistent with the presence of this residue in a loop in the proposed HuIFNalpha2 model. Based on this model structure potential receptor binding sites are identified. (C) 1993 Wiley-Liss, Inc.

引用

页码：62 / 74

页数：13

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