CDC7 PROTEIN-KINASE ACTIVITY IS REQUIRED FOR MITOSIS AND MEIOSIS IN SACCHAROMYCES-CEREVISIAE

被引：30

作者：

BUCK, V ^{[1
]}

WHITE, A ^{[1
]}

ROSAMOND, J ^{[1
]}

机构：

[1] UNIV MANCHESTER,DEPT BIOCHEM & MOLEC BIOL,MOLEC BIOL GRP,OXFORD RD,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 227卷 / 03期

关键词：

CDC7; SACCHAROMYCES-CEREVISIAE; PROTEIN KINASE; CELL CYCLE; DNA SYNTHESIS;

D O I：

10.1007/BF00273937

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The product of the CDC7 gene of Saccharomyces cerevisiae has multiple cellular functions, being needed for the initiation of DNA synthesis during mitosis as well as for synaptonemal complex formation and commitment to recombination during meiosis. The CDC7 protein has protein kinase activity and contains the conserved residues characteristic of the protein kinase catalytic domain. To determine which of the cellular functions of CDC7 require this protein kinase activity, we have mutated some of the conserved residues within the CDC7 catalytic domain and have examined the ability of the mutant proteins to support mitosis and meiosis. The results indicate that the protein kinase activity of the CDC7 gene product is essential for its function in both mitosis and meiosis and that this activity is potentially regulated by phosphorylation of the CDC7 protein.

引用

页码：452 / 457

页数：6

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