REGULATION OF A MAJOR CLONED VOLTAGE-GATED K+ CHANNEL FROM HUMAN LYMPHOCYTES-T

被引：33

作者：

ATTALI, B ^{[1
]}

HONORE, E ^{[1
]}

LESAGE, F ^{[1
]}

LAZDUNSKI, M ^{[1
]}

BARHANIN, J ^{[1
]}

机构：

[1] CNRS,INST PHARMACOL MOLEC & CELLULAIRE,660 ROUTE LUCIOLES,SOPHIA ANTIPOLIS,F-06560 VALBONNE,FRANCE

来源：

FEBS LETTERS | 1992年 / 303卷 / 2-3期

关键词：

T-CELL; JURKAT; SEROTONIN; KINASE-C; CALCIUM;

D O I：

10.1016/0014-5793(92)80526-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When expressed into Xenopus oocytes, HLK3 K+ channel (Kv1-3) induced a slowly inactivating voltage-dependent K+ current. We have studied the modulation of this K+ current by co-expressing a cloned 5-HT2 receptor together with HLK3 K+ channel protein. Application of 5-HT caused a long-lasting inhibition of the voltage-gated K+ current. This inhibitory modulation was mimicked by intracellular injection of inositol triphosphate or Ca2+ , as well as by incubation with phorbol esters or diacylglycerol analogs. Oocytes pretreatment with staurosporine and EGTA fully prevented 5-HT inhibitory action. Elevation of cAMP and cGMP levels into oocytes did not produce any detectable effect on the current recorded in the absence or the presence of 5-HT. These data suggest that the second messengers generated by phospholipase C activation may be important modulators of HLK3 K+ channels in the immune and the central nervous systems.

引用

页码：229 / 232

页数：4

共 32 条

[1]

ATTALI B, 1992, IN PRESS J BIOL CHEM

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