3-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN INTERFERON-GAMMA

被引：402

作者：

EALICK, SE

COOK, WJ

VIJAYKUMAR, S

CARSON, M

NAGABHUSHAN, TL

TROTTA, PP

BUGG, CE

机构：

[1] UNIV ALABAMA, CTR COMPREHENS CANC, BIRMINGHAM, AL 35294 USA

[2] UNIV ALABAMA, CTR MACROMOLEC CRYSTALLOG, DEPT PATHOL, BIRMINGHAM, AL 35294 USA

[3] SCHERING PLOUGH CORP, RES, BLOOMFIELD, NJ 07003 USA

[4] UNIV ALABAMA, CTR MACROMOLEC CRYSTALLOG, DEPT BIOCHEM, BIRMINGHAM, AL 35294 USA

来源：

SCIENCE | 1991年 / 252卷 / 5006期

关键词：

D O I：

10.1126/science.1902591

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is primarily alpha-helical, with six helices in each subunit that comprise approximately 62 percent of the structure; there is no beta-sheet. The dimeric structure of human interferon-gamma is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions.

引用

页码：698 / 702

页数：5

共 46 条

[1] 3-DIMENSIONAL STRUCTURE OF A GENETICALLY ENGINEERED VARIANT OF PORCINE GROWTH-HORMONE [J].