CPNPG METHYLATION IN MAMMALIAN-CELLS

被引：224

作者：

CLARK, SJ

HARRISON, J

FROMMER, M

机构：

[1] CSIRO, DIV BIOMOLEC ENGN, SYDNEY LAB, POB 184, N RYDE, NSW 2113, AUSTRALIA

[2] UNIV SYDNEY, SCH BIOL SCI, SYDNEY, NSW 2006, AUSTRALIA

来源：

NATURE GENETICS | 1995年 / 10卷 / 01期

关键词：

D O I：

10.1038/ng0595-20

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In vertebrate DNA, 3% to 5% of cytosine residues are present as 5-methylcytosine, and it is generally accepted that essentially all of this methylation occurs at cytosines which are contained in the symmetrical dinucleotide CpG. In this report we demonstrate, using bisulphite genomic sequencing, that the methylation machinery of mammalian cells is capable of both maintenance and de novo methylation at CpNpG sites. The existence of inherited CpNpG methylation in mammalian cells has important implications in gene regulation and in the aetiology of disease.

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页码：20 / 27

页数：8

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