INSECT VIRUS - ASSAYS FOR VIRAL REPLICATION AND PERSISTENCE IN MAMMALIAN-CELLS

被引：9

作者：

HARTIG, PC ^{[1
]}

CARDON, MC ^{[1
]}

KAWANISHI, CY ^{[1
]}

机构：

[1] NSI ENVIRONM SCI INC,RES TRIANGLE PK,NC

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 31卷 / 2-3期

关键词：

BACULOVIRUS; INSECT VIRUS; PERSISTENCE;

D O I：

10.1016/0166-0934(91)90171-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Viral pesticidal agents must be evaluated for potential health hazards prior to utilization. Assessment of the likelihood of replication in humans has included in vitro exposure of human cells to the potential pesticidal agent. Previous in vitro evaluation strategies have lacked positive controls. Thus, negative results, interpreted as no effect of the virus on human cells, could reflect basic deficiencies in the testing protocols. We designed a testing scheme for viral pesticides and used it to test the nuclear polyhedrosis virus of Autographa californica. Tests were aimed at evaluating potential replication or gene expression in primate cells. Parallel tests were run utilizing identical protocols with primate viruses known to produce the biological effect being evaluated. Thus protocols described were tested with positive viral controls.

引用

页码：335 / 344

页数：10

共 23 条

[1] PERSISTENT INFECTIONS IN L-CELLS WITH TEMPERATURE-SENSITIVE MUTANTS OF REOVIRUS [J].