DECREASED SIALIDASE ACTIVITY IN MONONUCLEAR LEUKOCYTES OF TYPE-1 DIABETIC SUBJECTS - RELATIONSHIP TO DIABETIC COMPLICATIONS AND GLYCEMIC CONTROL

被引：5

作者：

WATERS, PJ

FLYNN, MD

PENNOCK, CA

CORRALL, RJM

GREENWOOD, RJ

EISENTHAL, R

机构：

[1] PRINCE PHILIP DENT HOSP,LLANELLI SA14 8QF,DYFED,WALES

[2] UNIV BATH,DEPT BIOCHEM,BATH BA2 7AY,AVON,ENGLAND

来源：

DIABETIC MEDICINE | 1995年 / 12卷 / 08期

关键词：

SIALIDASE (= NEURAMINIDASE); SIALIC ACID; LYSOSOMES; GLYCOSIDE HYDROLASES; TYPE; 1; DIABETES; DIABETIC ANGIOPATHIES;

D O I：

10.1111/j.1464-5491.1995.tb00567.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Leucocyte surface sialic acid content influences surface charge, deformability, and leucocyte-endothelial interaction. Abnormal leucocyte structure and function contributes both to microvascular damage and diabetic complications. The aim of this study was to investigate altered leucocyte SA metabolism in diabetic subjects and measure lysosomal sialidase which regulates leucocyte surface sialylation. We examined 26 Type 1 (insulin-dependent) diabetic subjects with retinopathy, 26 Type 1 diabetic subjects without complications, and 38 matched normal control subjects. Sialidase was assayed in freshly prepared sonicates of pure mononuclear leucocytes (MNLs), using the fluorometric substrate 4-methyl-umbelliferyl-N-acetylneuraminic acid. In the subjects with diabetes there was a significant negative correlation between MNL sialidase activity and both HbA(1c) (r(s) = 0.37, p = 0.007) and fructosamine (r(s) = -0.31, p 0.026). MNL sialidase activity was significantly decreased in diabetic subjects with clinical evidence of complications compared to control subjects. HbA(1c) was significantly higher (p = 0.036) in diabetic patients with complications compared to those without. The observed decrease in MNL sialidase activity related to diabetic control may be important in the pathogenesis of vascular damage. Diabetes-associated changes in sialylation of functional cell surface glycoconjugates may have important clinical consequences.

引用

页码：670 / 673

页数：4

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