DECLINE WITH AGE OF THE RESPIRATORY-CHAIN ACTIVITY IN HUMAN SKELETAL-MUSCLE

被引：282

作者：

BOFFOLI, D ^{[1
]}

SCACCO, SC ^{[1
]}

VERGARI, R ^{[1
]}

SOLARINO, G ^{[1
]}

SANTACROCE, G ^{[1
]}

PAPA, S ^{[1
]}

机构：

[1] UNIV BARI,INST MED BIOCHEM & CHEM,I-70124 BARI,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1994年 / 1226卷 / 01期

关键词：

AGING; MITOCHONDRIAL RESPIRATION; RESPIRATORY CHAIN; CYTOCHROME C OXIDASE; NADH-UBIQUINONE OXIDOREDUCTASE; CYTOCHROME;

D O I：

10.1016/0925-4439(94)90061-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondrial respiratory systems have been screened in 63 othopaedic patients of age ranging between 17 and 91 years. The results show a statistically significant definite decrease with ageing of mitochondrial respiratory activity with pyruvate plus malate, succinate and ascorbate plus TMPD. This pattern is associated with an equally significant decrease with age of the enzymatic activity of complex I, II and IV. No significant decrease with age is, on the contrary, observed in the mitochondrial content of cytochromes a + a(3), and c + c(1). Preliminary Western blot analysis indicates an altered polypeptide pattern in cytochrome c oxidase. This study provides evidence for a decline with age of mitochondrial respiratory activity in human skeletal muscle, affecting complex I, II and IV.

引用

页码：73 / 82

页数：10

共 47 条

[1] MEASUREMENT OF CYTOCHROMES IN HUMAN SKELETAL-MUSCLE MITOCHONDRIA, ISOLATED FROM FRESH AND FROZEN STORED MUSCLE SPECIMENS [J].