ISOLATION AND CHARACTERIZATION OF CDNA CLONES FROM MOUSE SKELETAL-MUSCLE ACTIN MESSENGER-RNA

被引：30

作者：

LEADER, DP

GALL, I

CAMPBELL, P

FRISCHAUF, AM

机构：

[1] Department of Biochemistry, University of Glasgow, Glasgow

[2] European Molecular Biology Laboratory, D-6900 Heidelberg, FRG

来源：

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY | 1986年 / 5卷 / 03期

关键词：

D O I：

10.1089/dna.1986.5.235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence corresponding to approximately 98% of mouse skeletal muscle actin mRNA was determined from cDNA clones isolated from a library of recombinants in pBR322. One of these clones contains DNA corresponding to the complete amino acid coding region and a large part of the 5′ and 3′ untranslated regions of the mRNA. Comparison of the mouse coding region (conserved at the amino acid level) and noncoding regions with the corresponding regions of the rat skeletal muscle actin gene indicates that the noncoding regions have also been under selective pressure during evolution. © 1986, Mary Ann Liebert, Inc. All rights reserved.

引用

收藏

页码：235 / 238

页数：4

相关论文

共 18 条

[1] NUCLEOTIDE-SEQUENCE HETEROGENEITY AND SEQUENCE REARRANGEMENTS IN INFLUENZA-VIRUS CDNA [J].

FIELDS, S ;

WINTER, G .

GENE, 1981, 15 (2-3) :207-214

[2] THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE CHICK A-ACTIN GENE AND ITS EVOLUTIONARY RELATIONSHIP TO THE ACTIN GENE FAMILY [J].

FORNWALD, JA ;

KUNCIO, G ;

PENG, I ;

ORDAHL, CP .

NUCLEIC ACIDS RESEARCH, 1982, 10 (13) :3861-3876

[3] PROCOLLAGEN COMPLEMENTARY-DNA, A PROBE FOR MESSENGER-RNA PURIFICATION AND NUMBER OF TYPE-I COLLAGEN GENES [J].

FRISCHAUF, AM ;

LEHRACH, H ;

ROSNER, C ;

BOEDTKER, H .

BIOCHEMISTRY, 1978, 17 (16) :3243-3249

[4] 5'-CONFORMATION OF CAPPED ALFALFA MOSAIC-VIRUS RIBONUCLEIC ACID-4 MAY REFLECT ITS INDEPENDENCE OF THE CAP STRUCTURE OR OF CAP-BINDING PROTEIN FOR EFFICIENT TRANSLATION [J].

GEHRKE, L ;

AURON, PE ;

QUIGLEY, GJ ;

RICH, A ;

SONENBERG, N .

BIOCHEMISTRY, 1983, 22 (22) :5157-5164

[5] EVOLUTION OF THE HUMAN SARCOMERIC-ACTIN GENES - EVIDENCE FOR UNITS OF SELECTION WITHIN THE 3' UNTRANSLATED REGIONS OF THE MESSENGER-RNAS [J].

GUNNING, P ;

MOHUN, T ;

NG, SY ;

PONTE, P ;

KEDES, L .

JOURNAL OF MOLECULAR EVOLUTION, 1984, 20 (3-4) :202-214

[6] ISOLATION AND CHARACTERIZATION OF CDNA CLONES FOR HUMAN SKELETAL-MUSCLE ALPHA-ACTIN [J].

HANAUER, A ;

LEVIN, M ;

HEILIG, R ;

DAEGELEN, D ;

KAHN, A ;

MANDEL, JL .

NUCLEIC ACIDS RESEARCH, 1983, 11 (11) :3503-3516

[7] CONSTRUCTION OF RECOMBINANT PLASMIDS CONTAINING RAT MUSCLE ACTIN AND MYOSIN LIGHT CHAIN DNA-SEQUENCES [J].

KATCOFF, D ;

NUDEL, U ;

ZEVINSONKIN, D ;

CARMON, Y ;

SHANI, M ;

LEHRACH, H ;

FRISCHAUF, AM ;

YAFFE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (02) :960-964

[8] THE STRUCTURE OF A CLONED MOUSE GAMMA-ACTIN PROCESSED PSEUDOGENE [J].

LEADER, DP ;

GALL, I ;

LEHRACH, H .

GENE, 1985, 36 (03) :369-374

[9]

Maxam A M, 1980, Methods Enzymol, V65, P499

[10]

MAYER Y, 1984, NUCLEIC ACIDS RES, V12, P1100