SIMPLE AND EFFICIENT METHOD TO REMOVE RIBONUCLEASE CONTAMINATION FROM PANCREATIC DEOXYRIBONUCLEASE PREPARATIONS

被引：60

作者：

BRISON, O

CHAMBON, P

机构：

[1] INSERM, INST CHIM BIOL, RECH CANC UNITE, F-67200 STRASBOURG, FRANCE

[2] CNRS, FAC MED, CTR NEUROCHIM, F-67085 STRASBOURG, FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1976年 / 75卷 / 02期

关键词：

D O I：

10.1016/0003-2697(76)90094-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An affinity chromatography method which efficiently removes the contaminating RNase activity from commercial pancreatic DNase preparations is described. The new method is compared with the iodoacetate treatment previously used for the preferential inactivation of RNase.

引用

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页码：402 / 409

页数：8

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