LOCALIZED MEMBRANE DEPOLARIZATIONS AND LOCALIZED CALCIUM INFLUX DURING ELECTRIC FIELD-GUIDED NEURITE GROWTH

被引：153

作者：

BEDLACK, RS ^{[1
]}

WEI, MD ^{[1
]}

LOEW, LM ^{[1
]}

机构：

[1] UNIV CONNECTICUT, CTR HLTH, DEPT PHYSIOL, FARMINGTON, CT 06030 USA

来源：

NEURON | 1992年 / 9卷 / 03期

关键词：

D O I：

10.1016/0896-6273(92)90178-G

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Our study explores the mechanisms behind neurite galvanotropism. Using phase, differential interference contrast and ratiometric fluorescence microscopy, we reveal four responses of N1E-115 mouse neuroblastoma cells to 0.1-1.0 mV/mu-m uniform DC electric fields: cathode-directed neurite initiation and elongation, cathode-biased growth cone filopodial protrusions, transient cathode-localized calcium increases, and persistent cathode-localized membrane depolarizations. These newly demonstrated events are temporally and spatially correlated, suggesting that they are causally related. The calcium increases are prevented by calcium channel blockers and by the removal of extracellular calcium. We therefore propose that the observed field-induced membrane depolarizations activate voltage-dependent calcium channels, resulting in cathode-localized calcium influx. This, in turn, may initiate the observed cathode-biased growth cone filopodial protrusions, followed by the cathode-directed neurite elongation.

引用

页码：393 / 403

页数：11

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