DIODE-ARRAY DETECTION OF LOW-LEVEL CO-ELUTING SPECIES IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：4

作者：

ANDERSON, NH

GRAY, MR

HINDS, CJ

机构：

[1] Sterling Research Group, Alnwick, Northumberland NE66 2JH, Willowburn Avenue

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 8-12期

关键词：

CHROMATOGRAPHIC PEAK INTEGRITY ASSESSMENT; DIODE ARRAY SPECTROSCOPY; HPLC; ANTAZOLINE; DANAZOL; CO-ELUTING SPECIES;

D O I：

10.1016/0731-7085(90)80132-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive method for detecting low levels (0.5 and 1.0%, w/w) of co-eluting species in HPLC has been developed. This method is based on the subtraction of normalized peak up-slope and down-slope spectra from that of the apex. Visual inspection of the resultant "difference spectra" allows for a qualitative judgement regarding the integrity of the peak under consideration.

引用

页码：853 / 857

页数：5

共 5 条

[1] RECENT DEVELOPMENTS IN MULTICHANNEL, PHOTODIODE-ARRAY, OPTICAL LC DETECTION [J].