UP-REGULATION OF VASOPRESSIN MESSENGER-RNA IN PARAVENTRICULAR HYPOPHYSIOTROPHIC NEURONS AFTER ACUTE IMMOBILIZATION STRESS

被引：78

作者：

BARTANUSZ, V

AUBRY, JM

JEZOVA, D

BAFFI, J

KISS, JZ

机构：

[1] UNIV GENEVA,SCH MED,DEPT MORPHOL,CH-1211 GENEVA,SWITZERLAND

[2] IUPG,DEPT PSYCHIAT,GENEVA,SWITZERLAND

[3] SLOVAK ACAD SCI,INST EXPTL ENDOCRINOL,CS-80936 BRATISLAVA,SLOVAKIA

[4] SEMMELWEIS UNIV,SCH MED,DEPT ANAT 1,BUDAPEST,HUNGARY

来源：

NEUROENDOCRINOLOGY | 1993年 / 58卷 / 06期

关键词：

IN SITU HYBRIDIZATION; CORTICOTROPIN-RELEASING FACTOR; HYPOTHALAMIC PARAVENTRICULAR NUCLEUS;

D O I：

10.1159/000126602

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Cellular levels of vasopressin (VP) and corticotropin-releasing factor (CRF) mRNAs were determined in the rat hypothalamic paraventricular nucleus after acute immobilization stress. Messenger RNA levels were measured by in situ hybridization histochemistry using S-35-labeled synthetic DNA oligonucleotide probes and quantitative autoradiography. Analysis of histograms related to density values revealed that two hours after immobilization the average cellular VP and CRF mRNA levels were significantly increased (145 +/-30.3 and 68 +/- 21.3%, respectively, above control values). Moreover, the number of VP-expressing parvicellular neurons was doubled in stressed rats relative to controls. These results indicate that single acute immobilization stress is sufficient to up-regulate VP as well as CRF mRNA production in the hypothalamic CRF-neurosecretory system.

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页码：625 / 629

页数：5

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