ESTRADIOL-INDUCED SYNTHESIS OF VITELLOGENIN .3. ISOLATION AND CHARACTERIZATION OF VITELLOGENIN MESSENGER-RNA FROM AVIAN LIVER

被引：29

作者：

AB, G ^{[1
]}

ROSKAM, WG ^{[1
]}

DIJKSTRA, J ^{[1
]}

MULDER, J ^{[1
]}

WILLEMS, M ^{[1
]}

VANDERENDE, A ^{[1
]}

GRUBER, M ^{[1
]}

机构：

[1] STATE UNIV GRONINGEN, BIOCHEM LAB, GRONINGEN, NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1976年 / 454卷 / 01期

关键词：

D O I：

10.1016/0005-2787(76)90355-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mRNA of the hormone-induced protein vitellogenin was isolated from the liver of estrogen-treated roosters. Starting from total polysomal RNA, the vitellogenin messenger was purified 67-fold by oligo(dT)-cellulose [oligo(deoxythymidylic acid) cellulose] chromatography and sizing on a sucrose gradient. The messenger was translated in vitro into a 170,000 dalton polypeptide chain, having the immunochemical characteristics of vitellogenin. From electrophoretic and immunochemical analysis of the in vitro product of translation, at least 63% of the messenger activity of the RNA preparation could be attributed to vitellogenin mRNA. Gel electrophoresis of the most purified fraction revealed residual contamination with the larger ribosomal RNA species. The MW of the mRNA molecule, obtained by contour length measurements in the EM, lies between 2.5 .cntdot. 106-2.8 .cntdot. 106.

引用

页码：67 / 78

页数：12

共 26 条

[1]

BERGINK EW, 1973, BIOCHIM BIOPHYS ACTA, V294, P497, DOI 10.1016/0005-2787(73)90105-6

[2]

BERGINK EW, 1974, AM ZOOL, V14, P1177

[3]

BERGINK EW, 1974, J BIOL CHEM, V249, P2897

[4] CHARACTERIZATION OF POLYSOMES FROM XENOPUS LIVER SYNTHESIZING VITELLOGENIN AND TRANSLATION OF VITELLOGENIN AND ALBUMIN MESSENGER-RNAS INVITRO [J].

BERRIDGE, MV ;

FARMER, SR ;

GREEN, CD ;

HENSHAW, EC ;

TATA, JR .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 62 (01) :161-171

[5] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].

BONNER, WM ;

LASKEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88

[6] LIPOVITELLIN SYNTHESIZING POLYSOMES - SPECIFIC AND QUANTITATIVE ISOLATION [J].

BOS, ES ;

AB, G ;

VONK, RJ ;

GRUBER, M .

FEBS LETTERS, 1972, 24 (02) :197-+

[7] ELECTRON-MICROSCOPIC VISUALIZATION OF DELETION MUTATIONS [J].

DAVIS, RW ;

DAVIDSON, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (01) :243-&

[8]

DEELEY RG, 1975, J BIOL CHEM, V250, P9060

[9] DIRECT INDUCTION BY ESTRADIOL OF VITELLOGENIN SYNTHESIS IN ORGAN-CULTURES OF MALE XENOPUS-LAEVIS LIVER [J].

GREEN, CD ;

TATA, JR .

CELL, 1976, 7 (01) :131-139

[10] SYNTHESIS OF PHOSVITIN IN VITRO BY SLICES OF LIVER FROM LAYING HEN [J].

HEALD, PJ ;

MCLACHLAN, PM .

BIOCHEMICAL JOURNAL, 1965, 94 (01) :32-+

← 1 2 3 →