EVIDENCE FOR RIFAMPICIN-PROMOTED READTHROUGH OF A FULLY RHO-DEPENDENT TRANSCRIPTIONAL TERMINATOR

被引：11

作者：

CROMIE, KD ^{[1
]}

HAYWARD, RS ^{[1
]}

机构：

[1] UNIV EDINBURGH, DEPT MOLEC BIOL, EDINBURGH EH9 3JR, MIDLOTHIAN, SCOTLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1984年 / 193卷 / 03期

关键词：

D O I：

10.1007/BF00382095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rifampicin promotes readthrough of various transcriptional terminators in vivo, a conclusion based on previous measurements of galactokinase or .beta.-galactosidase synthesis in Escherichia coli strains with plasmids having the terminators fused upstream of galK or lacZ, respectively. However, the terminators tested did not include a fully rho-dependent signal. It is now shown that the drug stimulates galactokinase production when galK is fused downstream of such a terminator, the 1st rightward terminator of phage .lambda.. The nusA1 host mutation affects neither termination nor the drug response at this site.

引用

页码：532 / 534

页数：3

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