CELL-CYCLE CONTROL BY A COMPLEX OF THE CYCLIN HCS26 (PCL1) AND THE KINASE PHO85

The events of the eukaryotic cell cycle are governed by cyclin-dependent kinases (cdk's), whose activation requires association with cyclin regulatory subunits expressed at specific cell cycle stages. In the budding yeast Saccharomyces cerevisiae, the cell cycle is thought to be controlled by a single cdk, CDC28. Passage through the G(1) phase of the cell cycle is regulated by complexes of CDC28 and G(1) cyclins (CLN1, CLN2, and CLN3). A putative G(1) cyclin, HCS26, has recently been identified. In a/alpha diploid cells lacking CLN1 and CLN2, HCS26 is required for passage through G(1). HCS26 does not associate with CDC28, but instead associates with PHO85, a closely related protein kinase. Thus, budding yeast, like higher eukaryotes, use multiple cdk's in the regulation of cell cycle progression.