CHARACTERIZATION OF ANGIOGENIN RECEPTORS ON BOVINE BRAIN CAPILLARY ENDOTHELIAL-CELLS

被引：21

作者：

CHAMOUX, M

DEHOUCK, MP

FRUCHART, JC

SPIK, G

MONTREUIL, J

CECCHELLI, R

机构：

[1] INST PASTEUR,INSERM,U325,SERLIA,F-59019 LILLE,FRANCE

[2] UNIV SCI & TECH LILLE FLANDRES ARTOIS,F-59655 VILLENEUVE DASCQ,FRANCE

[3] UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB,CNRS,UNITE MIXTE RECH 111,F-59655 VILLENEUVE DASCQ,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(05)80261-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitogenic effect of bovine milk angiogenin was studied on bovine brain capillary and aortic endothelial cells, smooth muscle cells and fibroblasts. The proliferation of only bovine brain capillary endothelial cells was detected at concentrations ranging from 10 to 1,000 ng/ml, with a maximum effect at 100ng/ml. This mitogenic activity may be correlated with a specific binding of angiogenin which was demonstrated only to bovine brain capillary endothelial cells. [125I]-labeled angiogenin binding was time and concentration dependent and saturable. Scatchard analyses of binding data showed evidence of a single class of binding sites with an apparent dissociation constant of 5.10-10 M. The molecular mass of the angiogenin receptor (49 kDa) was determined by ligand blotting. © 1991 Academic Press, Inc.

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页码：833 / 839

页数：7

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