QUANTITATIVE-ANALYSIS OF ALKYLACYL, ALKENYLACYL, AND DIACYL TYPES OF DIGLYCERIDES OBTAINED FROM GLYCEROPHOSPHOLIPIDS

被引：13

作者：

FRANCESCANGELI, E ^{[1
]}

PORCELLATI, S ^{[1
]}

HORROCKS, LA ^{[1
]}

GORACCI, G ^{[1
]}

机构：

[1] OHIO STATE UNIV, DEPT PHYSIOL CHEM, COLUMBUS, OH 43210 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1987年 / 10卷 / 12期

关键词：

D O I：

10.1080/01483918708066827

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The 1-alkyl-2-acyl, 1-alk-1''-enyl-2-acyl, and 1,2-diacyl-sn-glycerol moieties of choline and ethanolamine glycerophospholipids were converted to the dinitrobenzoyl derivatives. These compounds were separated by high performance liquid chromatography and quantitated by their absorbance at 235 nm. Peak areas were proportional to the amount injected. Separations were optimal for the derivatives from 5 to 30 nmol of the glycerophospholipids. The compositions of ethanolamine glycerophospholipids from rat brain and of choline and ethanolamine glycerophospholipids from human platelets agreed well with previous results obtained with much longer procedures.

引用

页码：2799 / 2808

页数：10

共 15 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF DIGLYCERIDE PARA-NITROBENZOATES - AN APPROACH TO MOLECULAR ANALYSIS OF PHOSPHOLIPIDS [J].

BATLEY, M ;

PACKER, NH ;

REDMOND, JW .

JOURNAL OF CHROMATOGRAPHY, 1980, 198 (04) :520-525

[2]

BINAGLIA L, 1982, J LIPID RES, V23, P955

[3]

BLANK ML, 1975, BIOCHIM BIOPHYS ACTA, V380, P208, DOI 10.1016/0005-2760(75)90007-7

[4]

BLANK ML, 1984, J CHROMATOGR, V298, P473, DOI 10.1016/S0021-9673(01)92744-X

[5] SEPARATION OF PHOSPHOLIPIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - ALL MAJOR CLASSES, INCLUDING ETHANOLAMINE AND CHOLINE PLASMALOGENS, AND MOST MINOR CLASSES, INCLUDING LYSOPHOSPHATIDYLETHANOLAMINE [J].

DUGAN, LL ;

DEMEDIUK, P ;

PENDLEY, CE ;

HORROCKS, LA .

JOURNAL OF CHROMATOGRAPHY, 1986, 378 (02) :317-327

[6] STUDIES ON PHOSPHOLIPIDS OF RAT BRAIN WHICH CONTAIN GLYCERYL ETHERS [J].

HORROCKS, LA ;

ANSELL, GB .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 137 (01) :90-&

[7]

HORROCKS LA, 1972, RESEARCH METHODS NEU, V1, P223

[8]

Horrocks LA., 1982, New Comprehensive Biochemistry, V4, P51

[9] A SENSITIVE METHOD FOR QUANTITATIVE-ANALYSIS OF PHOSPHOLIPID MOLECULAR-SPECIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KITO, M ;

TAKAMURA, H ;

NARITA, H ;

URADE, R .

JOURNAL OF BIOCHEMISTRY, 1985, 98 (02) :327-331

[10] 1-0-ALKYL-LINKED PHOSPHOGLYCERIDES OF HUMAN-PLATELETS - DISTRIBUTION OF ARACHIDONATE AND OTHER ACYL RESIDUES IN THE ETHER-LINKED AND DIACYL SPECIES [J].

MUELLER, HW ;

PURDON, AD ;

SMITH, JB ;

WYKLE, RL .

LIPIDS, 1983, 18 (11) :814-819

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