C-MYC EXPRESSION IS REDUCED IN ANTIPAIN-TREATED PROLIFERATING C3H 10T1/2 CELLS

被引：42

作者：

CHANG, JD

BILLINGS, PC

KENNEDY, AR

机构：

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1985年 / 133卷 / 02期

关键词：

D O I：

10.1016/0006-291X(85)90979-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this report, we demonstrate that treatment of proliferating irradiated and nonirradiated C3H 10T1/2 cells with the protease inhibitor antipain is associated with a reduction in c-myc expression. Under conditions in which antipain treatment results in reduced c-myc transcripts, there is no effect on total RNA synthesis, growth rate, or saturation density. Antipain may be a useful inhibitor to which to further study the role of c-myc in cellular physiology.

引用

收藏

页码：830 / 835

页数：6

相关论文

共 25 条

[1] HOMOGENEOUSLY STAINING CHROMOSOMAL REGIONS CONTAIN AMPLIFIED COPIES OF AN ABUNDANTLY EXPRESSED CELLULAR ONCOGENE (C-MYC) IN MALIGNANT NEUROENDOCRINE CELLS FROM A HUMAN-COLON CARCINOMA [J].

ALITALO, K ;

SCHWAB, M ;

LIN, CC ;

VARMUS, HE ;

BISHOP, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (06) :1707-1711

[2] METHYLMERCURY AS A REVERSIBLE DENATURING AGENT FOR AGAROSE-GEL ELECTROPHORESIS [J].

BAILEY, JM ;

DAVIDSON, N .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :75-85

[3]

BILLINGS PC, 1985, UNPUB CANCER RES

[4] CELL-CYCLE CONTROL OF C-MYC BUT NOT C-RAS EXPRESSION IS LOST FOLLOWING CHEMICAL TRANSFORMATION [J].

CAMPISI, J ;

GRAY, HE ;

PARDEE, AB ;

DEAN, M ;

SONENSHEIN, GE .

CELL, 1984, 36 (02) :241-247

[5] AMPLIFICATION OF ENDOGENOUS MYC-RELATED DNA-SEQUENCES IN A HUMAN MYELOID-LEUKEMIA CELL-LINE [J].

COLLINS, S ;

GROUDINE, M .

NATURE, 1982, 298 (5875) :679-681

[6] CLOSE LINK BETWEEN REDUCTION OF C-MYC EXPRESSION BY INTERFERON AND G0/G1 ARREST [J].

EINAT, M ;

RESNITZKY, D ;

KIMCHI, A .

NATURE, 1985, 313 (6003) :597-600

[7]

Favaloro J, 1980, Methods Enzymol, V65, P718

[8] ACTIVATION OF A CELLULAR ONC GENE BY PROMOTER INSERTION IN ALV-INDUCED LYMPHOID LEUKOSIS [J].

HAYWARD, WS ;

NEEL, BG ;

ASTRIN, SM .

NATURE, 1981, 290 (5806) :475-480

[9] CELL-SPECIFIC REGULATION OF THE C-MYC-GENE BY LYMPHOCYTE MITOGENS AND PLATELET-DERIVED GROWTH-FACTOR [J].

KELLY, K ;

COCHRAN, BH ;

STILES, CD ;

LEDER, P .

CELL, 1983, 35 (03) :603-610

[10] TIMING OF THE STEPS IN TRANSFORMATION OF C3H 10T1/2 CELLS BY X-IRRADIATION [J].

KENNEDY, AR ;

CAIRNS, J ;

LITTLE, JB .

NATURE, 1984, 307 (5946) :85-86