IDENTIFICATION AND CHARACTERIZATION OF A MAJOR SCHISTOSOMA-MANSONI GLYCOPROTEIN ANTIGEN CROSS-REACTIVE WITH FASCIOLA-HEPATICA

被引：20

作者：

ARONSTEIN, WS ^{[1
]}

DALTON, JP ^{[1
]}

WEISS, JB ^{[1
]}

STRAND, M ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV, SCH MED, DEPT PHARMACOL & EXPTL THERAPEUT, 725 N WOLFE ST, BALTIMORE, MD 21205 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1985年 / 34卷 / 05期

关键词：

D O I：

10.4269/ajtmh.1985.34.879

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A major surface antigen of Schistosoma mansoni has been identified and characterized as a glycoprotein of 66,000 molecular weight (Mr) and isoelectric point of 6.2-6.1 (SM66-GP) by use of a monoclonal antibody. The antigen was expressed by schistosome eggs, cercariae, larvae, and adults, and was recognized by sera of schistosome infected hosts. Direct immunofluorescence microscopy showed the antigen was distributed in a uniform pattern on the entire worm surface. Indirect immunofluorescence microscopy revealed that it was present in the parenchymal tissue of immature and mature Fasciola hepatica, in the gut of the mature fluke, and in embryonated fasciola eggs. The cross-reactive F. hepatica epitope recognized was expressed on a polypeptide of Mr 220,000.

引用

页码：879 / 888

页数：10

共 38 条

[1] IDENTIFICATION OF SPECIES-SPECIFIC AND GENDER-SPECIFIC PROTEINS AND GLYCOPROTEINS OF 3 HUMAN SCHISTOSOMES [J].