TOWARDS CONSTRUCTION OF A HIGH-RESOLUTION MAP OF THE MOUSE GENOME USING PCR-ANALYZED MICROSATELLITES

被引：452

作者：

LOVE, JM ^{[1
]}

KNIGHT, AM ^{[1
]}

MCALEER, MA ^{[1
]}

TODD, JA ^{[1
]}

机构：

[1] CLIN RES CTR,TRANSPLANTAT BIOL SECT,HARROW HA1 3UJ,MIDDX,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 14期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/18.14.4123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, CA/GT, showed size variations between different inbred strains of mice and the wild mouse, Mus spretus. 62% of sequences had 3 or more alleles. GA/CT and AT/TA -containing sequences were also variable. About half of these size variants were detectable by agarose gel electrophoresis. This simple approach is extremely useful in linkage and genome mapping studies and will facilitate construction of high resolution maps of both the mouse and human genomes. © 1990 Oxford University Press.

引用

页码：4123 / 4130

页数：8

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