THE LINEAR PCR REACTION - A SIMPLE AND ROBUST METHOD FOR SEQUENCING AMPLIFIED RIBOSOMAL-RNA GENES

被引：216

作者：

EMBLEY, TM

机构：

[1] Department of Zoology, Natural History Museum, London, SW7 5BD, Cromwell Road

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1991年 / 13卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1991.tb00600.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The linear polymerase chain reaction was used to sequence amplified RNA genes from strains of Bacillus, Thermus and Legionella. The technique described is simple and reproducible and it works well with double standard product which has been PEG precipitated directly from PCR reactions.

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页码：171 / 174

页数：4

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