MOLECULAR-CLONING AND EXPRESSION OF A COMPLEMENTARY-DNA FOR INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE

被引：131

作者：

CHOI, KY

KIM, HK

LEE, SY

MOON, KH

SIM, SS

KIM, JW

CHUNG, HK

RHEE, SG

机构：

[1] NHLBI,BIOCHEM LAB,BETHESDA,MD 20892

[2] SEOUL NATL UNIV,COLL MED,DEPT BIOCHEM,SEOUL 151,SOUTH KOREA

来源：

SCIENCE | 1990年 / 248卷 / 4951期

关键词：

D O I：

10.1126/science.2157285

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A complementary DNA (cDNA) done that encodes inositol 1,4,5-trisphosphate 3-kinase was isolated from a rat brain cDNA expression library with the use of monoclonal antibodies. This clone had an open reading frame that would direct the synthesis of a protein consisting of 449 amino acids and with a molecular mass of 49,853 daltons. The putative protein revealed a potential calmodulin-binding site and six regions with amino acid compositions (PEST regions) common to proteins that are susceptible to calpain. Expression of the cDNA in COS cells resulted in an approximately 150-fold increase in inositol 1,4,5-trisphosphate 3-kinase activity of these cells.

引用

页码：64 / 66

页数：3

共 38 条

[1] INOSITOL PHOSPHATES AND CELL SIGNALING [J].