SEPARATION AND QUANTIFICATION OF HEART AND LIVER PHOSPHOLIPID CLASSES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A NEW LIGHT-SCATTERING DETECTOR

被引：51

作者：

JUANEDA, P

ROCQUELIN, G

ASTORG, PO

机构：

[1] Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, Dijon cedex

来源：

LIPIDS | 1990年 / 25卷 / 11期

关键词：

D O I：

10.1007/BF02544047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work describes a one-step separation of rat tissue phospholipid classes by high-performance liquid chromatography (HPLC) using a silica column and a new light-scattering detector (LSD). Complete separation of phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylethanolamine, and lysophosphatidylcholine was obtained. Direct quantification was achieved after detector calibration for each phospholipid class. The detector response was shown to be linear within the ranges used. The LSD results agreed well with those obtained by phospholipid phosphorus assay. The present method was applied to rat heart and rat liver phospholipid analysis. © 1985 American Oil Chemists' Society.

引用

页码：756 / 759

页数：4

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