RECOMBINANT THROMBOPOIETIN INDUCES RAPID PROTEIN-TYROSINE PHOSPHORYLATION OF JANUS KINASE-2 AND SHC IN HUMAN BLOOD-PLATELETS

被引：146

作者：

MIYAKAWA, Y

ODA, A

DRUKER, BJ

KATO, T

MIYAZAKI, H

HANDA, M

IKEDA, Y

机构：

[1] KEIO UNIV,SCH MED,DEPT INTERNAL MED,DIV HEMATOL,TOKYO 160,JAPAN

[2] OREGON HLTH SCI UNIV,DIV HEMATOL & MED ONCOL,PORTLAND,OR 97201

[3] KIRIN BREWERY CO LTD,PHARMACEUT RES LAB,MAEBASHI,GUMMA,JAPAN

[4] KEIO UNIV,CTR BLOOD,TOKYO 160,JAPAN

来源：

BLOOD | 1995年 / 86卷 / 01期

关键词：

D O I：

10.1182/blood.V86.1.23.bloodjournal86123

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A cDNA for the thrombopoietin has been cloned by several groups. The recombinant thrombopoietin has been reported to stimulate the megakaryocytopoiesis and thrombopoiesis. Little is known regarding the molecular basis of its effects. To elucidate the molecular mechanism involved in signal transduction, we have investigated the effects of thrombopoietin on platelet tyrosine phosphorylation. We report here that thrombopoietin induced time- and dose-dependent tyrosine phosphorylation of several proteins including Janus kinase 2 (Jak2) and a 52-kD protein, Shc, in human blood platelets. Both Jak2 and She were tyrosine phosphorylated within 15 seconds after stimulation. The tyrosine phosphorylation of Jak2 was accompanied by increased kinase activity, whereas Shc tyrosine phosphorylation induced its association with a 25-kD protein, Grb2. Thus, our data suggest that Jak2, Shc, and Grb2 may be involved in signal transduction after ligand binding to c-mpl in human platelets. (C) 1995 by The American Society of Hematology.

引用

页码：23 / 27

页数：5

共 30 条

[1] A DOMINANT-NEGATIVE ERYTHROPOIETIN (EPO) RECEPTOR INHIBITS EPO-DEPENDENT GROWTH AND BLOCKS F-GP55-DEPENDENT TRANSFORMATION [J].

BARBER, DL ;

DEMARTINO, JC ;

SHOWERS, MO ;

DANDREA, AD .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) :2257-2265

[2] IDENTIFICATION AND CLONING OF A MEGAKARYOCYTE GROWTH AND DEVELOPMENT FACTOR THAT IS A LIGAND FOR THE CYTOKINE RECEPTOR MPL [J].

BARTLEY, TD ;

BOGENBERGER, J ;

HUNT, P ;

LI, YS ;

LU, HS ;

MARTIN, F ;

CHANG, MS ;

SAMAL, B ;

NICHOL, JL ;

SWIFT, S ;

JOHNSON, MJ ;

HSU, RY ;

PARKER, VP ;

SUGGS, S ;

SKRINE, JD ;

MEREWETHER, LA ;

CLOGSTON, C ;

HSU, E ;

HOKOM, MM ;

HORNKOHL, A ;

CHOI, E ;

PANGELINAN, M ;

SUN, Y ;

MAR, V ;

MCNINCH, J ;

SIMONET, L ;

JACOBSEN, F ;

XIE, C ;

SHUTTER, J ;

CHUTE, H ;

BASU, R ;

SELANDER, L ;

TROLLINGER, D ;

SIEU, L ;

PADILLA, D ;

TRAIL, G ;

ELLIOTT, G ;

IZUMI, R ;

COVEY, T ;

CROUSE, J ;

GARCIA, A ;

XU, W ;

DELCASTILLO, J ;

BIRON, J ;

COLE, S ;

HU, MCT ;

PACIFICI, R ;

PONTING, I ;

SARIS, C ;

WEN, D .

CELL, 1994, 77 (07) :1117-1124

[3]

BOULTON TG, 1994, J BIOL CHEM, V269, P11648

[4]

CLARK EA, 1994, J BIOL CHEM, V269, P28859

[5]

DARNELL JE, 1994, SCIENCE, V268, P1415

[6] THE MPI RECEPTOR IS EXPRESSED IN THE MEGAKARYOCYTIC LINEAGE FROM LATE PROGENITORS TO PLATELETS [J].

DEBILI, N ;

WENDLING, F ;

COSMAN, D ;

TITEUX, M ;

FLORINDO, C ;

DUSANTERFOURT, I ;

SCHOOLEY, K ;

METHIA, N ;

CHARON, M ;

NADOR, R ;

BETTAIEB, A ;

VAINCHENKER, W .

BLOOD, 1995, 85 (02) :391-401

[7] STIMULATION OF MEGAKARYOCYTOPOIESIS AND THROMBOPOIESIS BY THE C-MPL LIGAND [J].

DESAUVAGE, FJ ;

HASS, PE ;

SPENCER, SD ;

MALLOY, BE ;

GURNEY, AL ;

SPENCER, SA ;

DARBONNE, WC ;

HENZEL, WJ ;

WONG, SC ;

KUANG, WJ ;

OLES, KJ ;

HULTGREN, B ;

SOLBERG, LA ;

GOEDDEL, DV ;

EATON, DL .

NATURE, 1994, 369 (6481) :533-538

[8]

DRACHMAN JG, 1994, BLOOD, V84, pA390

[9]

HAMMOND WP, 1994, BLOOD, V84, pA534

[10]

HE TC, 1994, J BIOL CHEM, V269, P18291

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