NANOSCALE SEPARATIONS COMBINED WITH TANDEM MASS-SPECTROMETRY

被引：26

作者：

DETERDING, LJ ^{[1
]}

MOSELEY, MA ^{[1
]}

TOMER, KB ^{[1
]}

JORGENSEN, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA, DEPT CHEM, CHAPEL HILL, NC 27514 USA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 554卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88438-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

High-efficiency separations of peptide mixtures, tryptic digests and other biological compounds have been achieved using nanoscale packed capillaries and capillary zone electrophoresis (CZE). The coaxial continuous-flow fast atom bombardment design is an excellent interface for coupling these separation techniques with mass spectrometry (MS). In addition, this interface is very useful for the acquisition of MS-MS data from compounds separated by nanoscale packed capillary liquid chromatography and CZE. Structurally informative daughter-ion spectra can be obtained at the low picomole to femtomole level.

引用

页码：73 / 82

页数：10

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