IDENTIFICATION OF MEMBRANE-PROTEINS OF HUMAN-BLOOD PLATELETS WITH A HYDROPHOBIC PHOTOLABEL

被引：4

作者：

BERKHOUT, TA ^{[1
]}

SCHIPHORST, ME ^{[1
]}

WIRTZ, KWA ^{[1
]}

SIXMA, JJ ^{[1
]}

机构：

[1] STATE UNIV UTRECHT, DEPT HAEMATOL, 3584 CH UTRECHT, NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1984年 / 778卷 / 02期

关键词：

D O I：

10.1016/0005-2736(84)90372-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A photoactivable glycolipid probe, 12-(4-azido-2-nitrophenoxy)stearoyl[1-14C]glucosamine, was used to label proteins and lipids of platelet membranes. The proteins were analyzed by two-dimensional high-resolution gel electrophoresis. The labeling patterns showed that 3 membrane proteins were labeled which were not previously identified by ectolabeling (Sixma et Schiphorst, 1980). Analysis of the lipid fraction showed that phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine were labeled by the probe. The distinct labeling of phosphatidylserine strongly suggests that the probe redistributes between the 2 halves of the bilayer.

引用

页码：298 / 304

页数：7

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