THE HUMAN INTERFERON-BETA GENE ENHANCER IS UNDER NEGATIVE CONTROL

被引：355

作者：

GOODBOURN, S ^{[1
]}

BURSTEIN, H ^{[1
]}

MANIATIS, T ^{[1
]}

机构：

[1] HARVARD UNIV, DEPT BIOCHEM & MOLEC BIOL, 7 DIVIN AVE, CAMBRIDGE, MA 02138 USA

来源：

CELL | 1986年 / 45卷 / 04期

关键词：

D O I：

10.1016/0092-8674(86)90292-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human .beta.-interferon gene is regulated by an inducible enhancer element. Analysis of the effect of deletions within this element on .beta.-interferon transcription indicates that this enhancer is under negative control. Deletion of sequences from the 3'' end of the enhancer leads to a dramatic increase in the basal level of .beta.-interferon mRNA and a decrease in the induction ratio. The remaining 5'' region of the enhancer can act as a strong constitutive transcription element, and it shares considerable homology with sequences known to be required for the activity of constitutive viral enhancers. We conclude that the .beta.-interferon enhancer consists of a constitutive transcription element and a negative regulatory sequence that prevents enhancer activity prior to induction. Thus, derepression of a constitutive transcription element appears to play a key role in the control of human .beta.-interferon gene expression.

引用

页码：601 / 610

页数：10

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