AEQUOREA GREEN FLUORESCENT PROTEIN - EXPRESSION OF THE GENE AND FLUORESCENCE CHARACTERISTICS OF THE RECOMBINANT PROTEIN

被引：322

作者：

INOUYE, S ^{[1
]}

TSUJI, FI ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,DIV MARINE BIOL RES 0202,LA JOLLA,CA 92093

来源：

FEBS LETTERS | 1994年 / 341卷 / 2-3期

关键词：

BIOLUMINESCENCE; AEQUORIN; CALCIUM; CHROMOPHORE; ENERGY TRANSFER;

D O I：

10.1016/0014-5793(94)80472-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the cDNA for Aequorea green fluorescent protein in E coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.

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页码：277 / 280

页数：4

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