THE YDP PLASMIDS - A UNIFORM SET OF VECTORS BEARING VERSATILE GENE DISRUPTION CASSETTES FOR SACCHAROMYCES-CEREVISIAE

被引：329

作者：

BERBEN, G

DUMONT, J

GILLIQUET, V

BOLLE, PA

HILGER, F

机构：

[1] Unité de Microbiologie, Faculté Des Sciences Agronomiques de Gembloux, Gembloux, B-5030

来源：

YEAST | 1991年 / 7卷 / 05期

关键词：

PLASMIDS; GENE DISRUPTION; SELECTABLE CASSETTES;

D O I：

10.1002/yea.320070506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The YDp plasmids (Yeast Disruption plasmids) are pUC9 vectors bearing a set of yeast gene disruption cassettes, all uniform in structure and differing only in the selectable marker used (HIS3, LEU2, LYS2, TRP1 or URA3). The markers, surrounded by translational termination codons, are embedded in the slightly modified sequence of the pUC9 multiple cloning sites.

引用

页码：475 / 477

页数：3

共 9 条

[1] GENETIC MANIPULATION OF SACCHAROMYCES-CEREVISIAE BY USE OF THE LYS2 GENE [J].