INTERTYPIC DIFFERENTIATION AND DETECTION OF INTRATYPIC VARIANTS AMONG CANINE AND PHOCID MORBILLIVIRUS ISOLATES BY KINETIC NEUTRALIZATION USING A NOVEL IMMUNOPLAQUE ASSAY

被引：27

作者：

HARDER, TC ^{[1
]}

KLUSMEYER, K ^{[1
]}

FREY, HR ^{[1
]}

ORVELL, C ^{[1
]}

LIESS, B ^{[1
]}

机构：

[1] NATL BACTERIOL LAB, S-10521 STOCKHOLM, SWEDEN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 41卷 / 01期

关键词：

CANINE DISTEMPER VIRUS; PHOCID DISTEMPER VIRUS; KINETIC NEUTRALIZATION; IMMUNOPLAQUE ASSAY; MONOCLONAL ANTIBODY;

D O I：

10.1016/0166-0934(93)90164-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intertypic antigenic differences and the intratypic variability of the closely related canine (CVD) and phocid distemper viruses (PDV) were examined using a molecular (monoclonal antibodies specific for the H- and F-glycoproteins) and a functional (kinetic neutralization, KN) approach. KN studies were carried out using a novel immunoplaque technique which combined conventional plaque assay and antigen-specific enzyme-immunostaining techniques. Morbillivirus isolates of canine and phocid origin clearly formed two separate groups. Minor antigenic differences were also evident within each cluster. A distemper isolate of mustelid origin was distinguishable from both CDV- and PDV-like prototype viruses by kinetic neutralization.

引用

页码：77 / 92

页数：16

共 47 条

[1] ROLE OF VIRUS-STRAIN IN CONVENTIONAL AND ENHANCED MEASLES PLAQUE NEUTRALIZATION TEST [J].