PHOSPHATIDYLINOSITOL HYDROLYSIS BY HUMAN-PLASMA PHOSPHOLIPASE-D

被引：16

作者：

BALSINDE, J ^{[1
]}

MOLLINEDO, F ^{[1
]}

机构：

[1] CSIC,CTR INVEST BIOL,VELAZQUEZ 144,E-28006 MADRID,SPAIN

来源：

FEBS LETTERS | 1990年 / 259卷 / 02期

关键词：

Human plasma; Phosphatidylinositol; Phospholipase D;

D O I：

10.1016/0014-5793(90)80017-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phospholipase D activity able to hydrolyze phosphatidylinositol has previously been described in the cytosol of human neutrophils. The experiments reported here demonstrate that this phosphatidylinositol-hydrolyzing phospholipase D activity is also present in human plasma. This activity was assessed by free inositol release from phosphatidylinositol substrate, by phosphatidate formation and by phosphatidylethanol formation through its capacity of catalyzing a transphosphatidylation reaction. This plasma enzyme activity shows an optimum pH of 8.0 and is inhibited by EGTA. © 1990.

引用

页码：237 / 240

页数：4

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