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DISTINCT CELLULAR EXPRESSION OF CALCINEURIN A-ALPHA AND A-BETA IN RAT-BRAIN

被引：130
作者：
KUNO, T
MUKAI, H
ITO, A
CHANG, CD
KISHIMA, K
SAITO, N
TANAKA, C
机构：
[1] Department of Pharmacology, Kobe University School of Medicine, Kobe
来源：
JOURNAL OF NEUROCHEMISTRY | 1992年 / 58卷 / 05期
关键词：
CALCINEURIN; CATALYTIC SUBUNIT; DISTRIBUTION OF ISOFORMS; PROTEIN PHOSPHATASE; CALMODULIN-BINDING PROTEIN;
D O I：
10.1111/j.1471-4159.1992.tb10036.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Specific polyclonal antibodies that distinguish the two distinct isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase, calcineurin A-alpha and A-beta, were prepared, and the distribution of calcineurin A-alpha and A-beta in rat brain was studied using immunochemical and immunocytochemical techniques. Immunochemical measurement revealed that the regional distributions of the two isoforms differed and that A-alpha was more abundant than A-beta in the rat brain. The subcellular distribution patterns of both isoforms were similar. Both isoforms were highly enriched in cytosolic fractions, including the synaptosomal cytosol. Immunocytochemical analysis revealed that both A-alpha and A-beta immunoreactivities differed in regional and cellular localizations. These different patterns of expression suggest that the two isoforms of calcineurin A may each have specific functions in modulating neuronal activity in particular cell types.
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页码：1643 / 1651
页数：9
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