ACTIVATED MICROGLIA INHIBIT MULTIPLICATION OF TOXOPLASMA-GONDII VIA A NITRIC-OXIDE MECHANISM

被引：138

作者：

CHAO, CC

ANDERSON, WR

HU, SX

GEKKER, G

MARTELLA, A

PETERSON, PK

机构：

[1] MINNEAPOLIS MED RES FDN INC,ELECTRONMICROSCOPY LAB,MINNEAPOLIS,MN 55404

[2] HENNEPIN CTY MED CTR,DEPT MED,MINNEAPOLIS,MN 55415

[3] HENNEPIN CTY MED CTR,DEPT PATHOL,MINNEAPOLIS,MN 55415

[4] UNIV MINNESOTA,SCH MED,MINNEAPOLIS,MN 55415

来源：

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1993年 / 67卷 / 02期

关键词：

D O I：

10.1006/clin.1993.1062

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The role of microglia in host defense against Toxoplasma gondii is unknown. In the present study, we investigated the multiplication of T. gondii tachyzoites in murine microglial cell cultures. T. gondii multiplied readily in these cells; multiplication was prevented when microglia were activated with interferon-γ plus lipopolysaccharide, a treatment that also upregulates nitric oxide (NO) synthase activity. Simultaneous treatment of microglial cell cultures with activation signals and the NO synthase inhibitor NG-monomethyl-Larginine (NGMA) prevented the antitoxoplasmic activity. Transmission electron microscopic analysis demonstrated degenerative tachyzoites in activated microgloa but not in control or NGMA groups. These findings support the view that the host defense function of activated microglia against T. gondii involves generation of the free radical NO. © 1993 Academic Press, Inc.

引用

页码：178 / 183

页数：6

共 25 条

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