EXPRESSION CLONING OF A RAT-BRAIN SOMATOSTATIN RECEPTOR CDNA

被引：225

作者：

KLUXEN, FW

BRUNS, C

LUBBERT, H

机构：

[1] Preclinical Research, Sandoz Pharma Ltd.

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 10期

关键词：

SOMATOSTATIN-14; SOMATOSTATIN-28; SMS-(201-995); G-PROTEIN-COUPLED RECEPTOR;

D O I：

10.1073/pnas.89.10.4618

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have used an expression-cloning strategy to isolate a cDNA encoding a somatostatin (somatotropin release-inhibiting factor, SRIF) receptor from rat cortex and hippocampus. A positive clone was identified by autoradiography after binding of radiolabeled SRIF to COS-1 cells previously transfected with pools of cDNA clones. The deduced amino acid sequence of the receptor displays sequence and structural homology to the family of G-protein-coupled receptors. The affinity of various SRIF analogs to the expressed receptor resembles their effects on growth hormone release from pituitary cells. In addition, the distribution of the mRNA in various tissues corresponds to that described for native SRIF receptors. Therefore, we conclude that we have isolated a rat brain SRIF receptor cDNA.

引用

页码：4618 / 4622

页数：5

共 51 条

[1] OPTIMIZATION OF ELECTROPORATION FOR TRANSFECTION OF MAMMALIAN-CELL LINES [J].