PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES

被引：625

作者：

COCKCROFT, S ^{[1
]}

THOMAS, GMH ^{[1
]}

FENSOME, A ^{[1
]}

GENY, B ^{[1
]}

CUNNINGHAM, E ^{[1
]}

GOUT, I ^{[1
]}

HILES, I ^{[1
]}

TOTTY, NF ^{[1
]}

TRUONG, Q ^{[1
]}

HSUAN, JJ ^{[1
]}

机构：

[1] UCL, SCH MED, LUDWIG INST CANC RES, LONDON W1P 8BT, ENGLAND

来源：

SCIENCE | 1994年 / 263卷 / 5146期

基金：

英国惠康基金;

关键词：

D O I：

10.1126/science.8290961

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on the cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation of phospholipase D in HL60 cells depleted of their cytosol by permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 and ARF3. Recombinant ARF1 substituted for purified ARF proteins in the reconstitution assay. These results indicate that phospholipase D is a downstream effector of ARF1 and ARF3. The well-established role of ARF in vesicular traffic would suggest that alterations in lipid content by PLD are an important determinant in vesicular dynamics.

引用

页码：523 / 526

页数：4

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