CRYSTALLIZATION OF MITOCHONDRIAL UBIQUINOL CYTOCHROME-C REDUCTASE

被引：42

作者：

YUE, WH ^{[1
]}

ZOU, YP ^{[1
]}

YU, L ^{[1
]}

YU, CA ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,OKLAHOMA AGR EXPT STN,DEPT BIOCHEM,STILLWATER,OK 74078

来源：

BIOCHEMISTRY | 1991年 / 30卷 / 09期

关键词：

D O I：

10.1021/bi00223a002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquinol-cytochrome c reductase of beef heart mitochondria was crystallized in the presence of decanoyl-N-methylglucamide, heptanetriol, and sodium chloride with poly(ethylene glycol) as precipitant. The largest crystal has dimensions of 4 X 2 X 1 mm. The crystalline enzyme is composed of 10 subunits. It contains 2.5 nmol of ubiquinone, 8.4 nmol of cytochrome b, 4.2 nmol of cytochrome c1, 4.2 nmol of iron-sulfur cluster, and 140 nmol of phospholipid per milligram of protein. Of the last, 36% is with diphosphatidylglycerol. The crystals are very stable in the cold and show full enzymatic activity when redissolved in aqueous solution. Absorption spectra of the redissolved crystals show a Soret to UV ratio of 0.88 and 1.01 in the oxidized and the reduced forms, respectively.

引用

页码：2303 / 2306

页数：4

共 25 条

[1]

AMES BN, 1960, J BIOL CHEM, V235, P769

[2] POLYPEPTIDE COMPOSITION OF UBIQUINONE-CYTOCHROME-C REDUCTASE (COMPLEX-III) FROM BEEF-HEART MITOCHONDRIA [J].

BELL, RL ;

CAPALDI, RA .

BIOCHEMISTRY, 1976, 15 (05) :996-1001

[3] REACTION OF ANTIMYCIN WITH A CYTOCHROME-B PREPARATION ACTIVE IN RECONSTITUTION OF RESPIRATORY CHAIN [J].

BERDEN, JA ;

SLATER, EC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 216 (02) :237-&

[4] TOPOLOGY OF PEPTIDES IN FREE AND MEMBRANE-BOUND COMPLEX-III (UBIQUINOL-CYTOCHROME C REDUCTASE) AS REVEALED BY LACTOPEROXIDASE AND PARA-DIAZONIUMBENZENE[S-35]SULFONATE LABELING [J].

GELLERFORS, P ;

NELSON, BD .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 80 (01) :275-282

[5] SUBUNIT ARRANGEMENT IN BEEF-HEART COMPLEX-III [J].

GONZALEZHALPHEN, D ;

LINDORFER, MA ;

CAPALDI, RA .

BIOCHEMISTRY, 1988, 27 (18) :7021-7031

[6]

HATEFI Y, 1985, ANNU REV BIOCHEM, V54, P1015, DOI 10.1146/annurev.bi.54.070185.005055

[7] N-D-GLUCO-N-METHYLALKANAMIDE COMPOUNDS, A NEW CLASS OF NON-IONIC DETERGENTS FOR MEMBRANE BIOCHEMISTRY [J].

HILDRETH, JEK .

BIOCHEMICAL JOURNAL, 1982, 207 (02) :363-366

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[10] EPR CHARACTERIZATION OF THE CYTOCHROME-B-C1 COMPLEX FROM RHODOBACTER-SPHAEROIDES [J].

MCCURLEY, JP ;

MIKI, T ;

YU, L ;

YU, CA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1020 (02) :176-186

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